Plates for thin - layer chromatography ( tlc ) with an attached layer of porous polymer monolith have been prepared and used for the separation of small molecules , peptides , and proteins 制備了涂覆有整體多孔聚合涂層的薄層色譜( tlc )板,并將其用于小分子、肽段和蛋白質(zhì)的分離。
Second , we used high performance liquid chromatography ( hplc ) , thin - layer chromatography ( tlc ) , gel chromatography ( gc ) , and protein and nucleic acid analytic apparatus to evaluate f i " s purification 其次,利用高效液相色譜,蛋白核酸分析儀,薄層層析,凝膠色譜等檢測手段,從各個角度入手,證明該f為單一組分。
Extract the bursin from the bursa fabricius of chichens and ducks through the ultrafilter with loooda filter . ( the bursin of chickens - cbs . the bursin of ducks - dbs ) determine the major constitution by high - performance liquid chromatography ( hplc ) and thin - layer chromatography ( tlc ) . it is proved that there is bursin ( bs ) in two extractions . caculate the quantitation of bursin in the extraction of the bursa fabricius of chickens and ducks . it can be found through tlc that the quantitation of cbs in the extraction of the bursa fabricius of chickens is 1 . 436mg / ml and the quantitation of dbs in the extraction of the bursa fabricius of ducks is 4 . 035mg / ml . the result obtained through hplcis that the quantitation of cbs is 1 . 199mg / ml and dbs is 3 . 316mg / ml 采用超濾法從正常雞、鴨法氏囊組織中提取分子量小于1000da的物質(zhì),分別以薄層層析法和高壓液相色譜法檢測其主要成分,證明兩種超濾液中均有法氏囊活性肽(囊素三肽, bursin , bs )的存在,分別計算雞、鴨法氏囊提取物中的囊素三肽的含量。薄層層析方法求得雞法氏囊提取物中的bs含量為1 . 436mg / ml ,鴨法氏囊提取物中的bs含量為4 . 035mg / ml ;高壓液相色譜的方法求得雞法氏囊提取物中的貼含量為1 . 199mg / ml ,鴨法氏囊提取物中bs含量為3 . 316mg / ml 。